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DNA Replication Worksheet

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DNA Replication Worksheet

DNA double helix structure

📚 Part 1: Multiple Choice

1. DNA replication occurs during which phase of the cell cycle?

G1 phase

S phase

G2 phase

M phase

2. Which enzyme is responsible for "unzipping" the DNA double helix?

DNA polymerase

DNA ligase

Helicase

Primase

3. DNA replication is described as semiconservative because:

Both strands are completely new

Each new DNA molecule contains one original and one new strand

Only half the DNA is replicated

The process conserves energy

4. Which of the following are required for DNA replication? (Select all that apply)

DNA template

DNA polymerase

RNA primer

ATP for energy

Ribosomes

🏷️ Part 2: Diagram Labelling

5. Label the diagram below showing DNA replication. Use the terms provided in the box.

Terms: Leading strand, Lagging strand, DNA polymerase, Helicase, Okazaki fragments, RNA primer, Replication fork

6. Fill in the blanks to complete the DNA replication process:

The enzyme _____________ unwinds the DNA double helix at the _____________. DNA synthesis occurs in the _____________ direction from 5' to 3'. The _____________ strand is synthesised continuously, while the _____________ strand is synthesised in short fragments called _____________.

✏️ Part 3: Short Answer Questions

7. Explain why DNA replication is essential for cell division.
8. Describe the role of DNA polymerase in DNA replication and explain why it can only add nucleotides in one direction.
9. Compare and contrast the synthesis of the leading strand and lagging strand during DNA replication.

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