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Cellular Respiration Investigation

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Cellular Respiration Investigation

A food-science team is testing how sugar concentration affects carbon dioxide production by yeast. Use the method and results below to analyse the investigation. Answer in complete sentences where appropriate. Treat the results as experimental data, not as measurements you collected yourself.

Investigation brief

Each flask contained 20 mL of 5% yeast suspension. The team added 20 mL of glucose solution at one of three concentrations: 0%, 2.5% or 5%. They kept the flasks at 35°C for 20 minutes and measured the carbon dioxide collected. Each concentration was tested three times. The team used identical flasks and the same yeast suspension throughout. Their question was: How does glucose concentration affect the rate of carbon dioxide production by yeast?

Glucose concentration

Trial 1

Trial 2

Trial 3

0%

2

1

2

2.5%

18

20

19

5%

34

37

35

Analyse the evidence

Use the results table to answer Questions 1–8.

1.Which variable did the team deliberately change, and which variable did they measure?
2.Calculate the mean carbon dioxide volume for the 2.5% glucose condition. Show your working.
3.Which glucose concentration had the highest mean carbon dioxide production? Give the mean and explain how you worked it out.
4.Choose the conclusion best supported by these results.
  • Increasing glucose concentration from 0% to 5% was associated with more carbon dioxide being produced.
  • Glucose concentration had no effect on carbon dioxide production.
  • Yeast produced the most carbon dioxide when no glucose was added.
  • The results prove that carbon dioxide production will keep increasing at every glucose concentration.
5.The team kept temperature, yeast amount, solution volume and trial time the same. Explain why controlling these variables makes the comparison fairer.
6.Identify one limitation or possible source of error in this method, and suggest a practical improvement.
7.Yeast can respire glucose anaerobically when oxygen is limited. Write the word equation for alcoholic fermentation in yeast and name the gas measured in this investigation.
8.The 5% condition produced more gas than the 2.5% condition, but this experiment tested no concentrations above 5%. What can the team reasonably predict about higher concentrations, and why should they avoid claiming certainty?

2 printable pages

  • Cellular Respiration Investigation, page 1 of 2: Investigation brief, Analyse the evidence

    Page 1

  • Cellular Respiration Investigation, page 2 of 2: 4. Choose the conclusion best supported by these results.

    Page 2

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