Scientific Investigation Report
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Scientific Investigation Report
Investigation details and results
Question: How does the temperature of the reaction mixture affect the volume of oxygen produced by potato catalase in 60 seconds? The student placed 2.0 g of potato pieces and 20 mL of hydrogen peroxide in a flask. The reaction mixture was brought to the target temperature in a water bath. Oxygen volume was recorded after 60 s. Three trials were completed at each temperature. The same potato mass, peroxide volume and concentration, reaction time, and potato source were used throughout.
Temperature (°C)
Trial 1 (mL)
Trial 2 (mL)
Trial 3 (mL)
10
8
9
7
20
18
20
19
30
31
33
32
40
24
22
23
50
10
8
9
Build the report
A scientific report communicates what was investigated, how it was tested, what the evidence shows, and how confidently the findings can be interpreted. Keep results factual; explain patterns and limitations in the discussion or conclusion.
- Methods
- Introduction
- Results
- Conclusion
- The lower reading at 50°C may be because the enzyme’s structure changed.
- At 30°C, the three oxygen readings were 31, 33 and 32 mL.
- A water bath would improve control of temperature in a repeat test.
- The results support a peak reaction rate near 30°C.
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