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Chromatography Model Exploration Worksheet

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Chromatography Model Exploration Worksheet

A lab team wants to find out whether a marker’s color is one substance or a mixture. Read the model, then solve the investigation questions. No experiment materials are needed. Word bank: mobile phase, stationary phase, solvent, ink, paper.

A Paper-Chromatography Model

In paper chromatography, a spot of ink is placed near the bottom of paper. The paper’s lower edge sits in a shallow solvent, with the ink spot above the liquid. The solvent travels up through the paper. It dissolves some ink components and carries them along. Components that dissolve more readily in the solvent and are less attracted to the paper usually travel farther. Different components can form separate colored bands. The solvent is called the mobile phase because it moves; the paper is the stationary phase because it stays in place. This model separates parts of a mixture—it does not create new colors. Different substances can sometimes produce similar band patterns, so matching patterns are useful evidence but do not prove identity by themselves.

1.What is the main purpose of paper chromatography in the model?
  • To create a new substance
  • To separate components of a mixture
  • To make the solvent disappear
  • To turn paper into ink
2.In the model, which material is the mobile phase, and why is it given that name?
3.The ink spot must start above the solvent level. What would likely happen if the spot were submerged at the start?
  • The ink could dissolve directly into the solvent instead of separating on the paper
  • The paper would become the mobile phase
  • The solvent would stop moving
  • The ink would become a pure substance
4.True or false: A colored band traveling farther proves that its component has a greater mass than every other component.
TrueFalse
5.Fill in the blanks using the word bank: mobile phase · stationary phase. The travels, while the stays in place.
6.A black marker sample separates into blue, yellow, and pink bands. What does this evidence suggest about the black ink?
7.Two components separate in the same solvent. Component A forms a band near the starting spot, while component B travels much farther. Give one reasonable explanation for their different distances.
8.A student changes both the paper type and the solvent at the same time. Why would it be difficult to tell which change caused a different band pattern?
9.Design a fair comparison to test whether two solvents separate the same marker ink differently. State what you would keep the same and what you would change.
10.A lab team wants to identify an unknown ink by comparing its band pattern with known inks. What should the team do, and why might a matching pattern be useful evidence rather than absolute proof of identity?

2 printable pages

  • Chromatography Model Exploration Worksheet, page 1 of 2: A Paper-Chromatography Model

    Page 1

  • Chromatography Model Exploration Worksheet, page 2 of 2: 5. Fill in the blanks using the word bank: mobile phase · stationary phase. The

    Page 2

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